Modified DOP-PCR for improved STR typing of degraded DNA from human skeletal remains and bloodstains
نویسندگان
چکیده
منابع مشابه
Duplex Alu Screening for Degraded DNA of Skeletal Human Remains
The human-specific Alu elements, belonging to the class of Short INterspersed Elements (SINEs), have been shown to be a powerful tool for population genetic studies. An earlier study in this department showed that it was possible to analyze Alu presence/absence in 3000-year-old skeletal human remains from the Bronze Age Lichtenstein cave in Lower Saxony, Germany. We developed duplex Alu screeni...
متن کاملDNA typing from skeletal remains: evaluation of multiplex and megaplex STR systems on DNA isolated from bone and teeth samples.
AIM To evaluate the performance of three multiplex short tandem repeat (STR) systems (AmpflSTR Profiler, AmpflSTR Profiler Plus, and AmpflSTR COfiler), and a megaplex STR system (PowerPlex 16) on DNA extracted from the skeletal remains. By performing a microbial DNA challenge study, we also evaluated the influence of microbial DNA on human DNA typing. METHODS A subset of 86 DNA extracts isola...
متن کاملSTR-typing of ancient skeletal remains: which multiplex-PCR kit is the best?
AIM To comparatively test nine co mmercially available short tandem repeat (STR)-multiplex kits (PowerPlex 16, 16HS, ES, ESI17, ESX17, S5 [all Promega]; AmpFiSTR Identifiler, NGM and SEfiler [all Applied Biosystems]) for their efficiency and applicability to analyze ancient and thus highly degraded DNA samples. METHODS Fifteen human skeletal remains from the late medieval age were obtained an...
متن کاملStr-typing of Nuclear Dna from Human Fecal Matter
Human fecal matter is routinely encountered in forensic casework—identifying the source of this material could link a suspect or victim to a crime scene. Previous methods used to identify the source of the fecal matter have primarily involved microscopic comparisons. While these methods can be useful, two sources of fecal matter are required, and typically the analysis cannot individualize the ...
متن کاملSkeletal remains presumed submerged in water for three years identified using PCR-STR analysis.
We describe the successful identification of the remains of a saponified body found in a dam by typing of nuclear DNA. Whereas DNA extracted from soft tissues yielded negative PCR results, DNA extracted from the bone by a slightly modified Qiagen procedure allowed the typing of sex (AMG locus) and of 10 additional STR loci. An identity document was found belonging to a man missing for 3 years a...
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ژورنال
عنوان ژورنال: Legal Medicine
سال: 2016
ISSN: 1344-6223
DOI: 10.1016/j.legalmed.2015.10.013